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This is certainly especially genuine for immunohistochemistry wherein ten% NBF continues to be shown to be a bad fixative (Fig. two). Some problems with the usage of ten% NBF like a fixative happen to be corrected through the introduction of other experimental techniques including antigen retrieval (Shi et al. 2001) and using tiny amplicons in RT-Q
The aforementioned methods of tissue processing may be attained in either a guide or an automatic style. Guide processing is time-consuming, Using the duration of most guide protocols exceeding the size of a daily function day.At the end of the operate, select the right prompt within the processor Show to empty the molten paraffin from the retort.P
This is often especially true for immunohistochemistry where ten% NBF continues to be revealed being a poor fixative (Fig. 2). Some problems with using 10% NBF as a fixative have already been corrected via the introduction of other experimental strategies like antigen retrieval (Shi et al. 2001) and the usage of smaller amplicons in RT-Q-PCR (Steg